SPHK1 antibody (AA 176-189)

Catalog No. ABIN343006

Product Details anti-SPHK1 Antibody

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Binding Specificity: AA 176-189

Reactivity: Human, Mouse, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human SPHK1. This antibody is expected to recognise all three reported isoforms (NP_068807.2, NP_892010.2, NP_001136073.1). Reported variants represent identical protein (NP_001136073.1, NP_001136074.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Hamster (100%) Rat, Panda, Dog, Bovine, Rabbit, Platypus (93%) Bat, Horse, Pig, Slime mold (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DVDLESEKYRRLGE from an internal region of human SPHK1 (NP_068807.2, NP_892010.2, NP_001136073.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Hamster (100%), Rat, Panda, Dog, Bovine, Rabbit, Platypus (93%), Bat, Horse, Pig, Slime mold (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000, 2 - 3 μg/mL), WB (2 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 40 kD band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kD according to NP_068807.2). Recommended concentration: 2-3 μg/mL.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK / SPHK1 (SPHK1 Products)

Synonyms: CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody

Background: Name/Gene ID: SPHK1
Subfamily: Sphingosine
Family: Non-protein Kinase

Synonyms: SPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK

Gene ID: 8877

NCBI Accession: NP_068807, NP_892010, NP_001136073

UniProt: Q9NYA1

Pathways: VEGF Signaling