KRT8 antibody

Catalog No. ABIN356298

Product Details anti-KRT8 Antibody

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes all simple epithelia and glandular (thyroid, breast, GI tract, respiratory tract, urinary tract). Most squamous carcinomas and all adenocarcinomas are stained by this antibody and keratinizing squamous carcinomas are mostly negative.
Cellular Localization: Cytoplasmic.

Cross-Reactivity (Details): Also reacts to: KRT18

Characteristics: Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A, Keratin, type IIcytoskeletal 8

Purification: Protein A Chromatography.

Immunogen: Purified keratins. Remarks: Molecular weight of antigen: CK8 is 52. 5 kDa and CK18 is 45 kDa.

Clone: CK207

Isotype: IgG2a

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence. Recommended Positive Control: Human breast, any GI tissue or skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Storethe antibody undiluted at 2-8 °Cfor one month or (in aliquots) at -28 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8 (KRT8 Products)

Synonyms: CARD2 antibody, CK-8 antibody, CK8 antibody, CYK8 antibody, K2C8 antibody, K8 antibody, KO antibody, AA960620 antibody, AL022697 antibody, AU019895 antibody, Card2 antibody, EndoA antibody, Krt-2.8 antibody, Krt2-8 antibody, CYKER antibody, KRT2-8 antibody, KERATIN8 antibody, ck8 antibody, cyk8 antibody, k2c8 antibody, card2 antibody, krt2-5 antibody, MGC69490 antibody, KRT8 antibody, DreK8 antibody, cb186 antibody, krt2-8 antibody, sb:cb186 antibody, wu:fa20h05 antibody, wu:fa95h10 antibody, wu:fb96c06 antibody, zf-K8 antibody, zfk8 antibody, DKFZp468F2127 antibody, keratin 8 antibody, keratin 8 S homeolog antibody, KRT8 antibody, Krt8 antibody, krt8.S antibody, krt8 antibody

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gene ID: 3856

UniProt: P05787