This antibody recognizes the recombinant soluble VEGFR-1/FLT-1 (native) but not the recombinant soluble VEGFR-1/FLT-1 (D1-5) both produced in Insects cells. Because of the different glycosylation of insect cells compared to mammalian cells the MW of sFlt-1 (native) is about 96 kDa instead of about 110 kDa of the naturally occurring sFlt-1. The antibody did not cross react with recombinant Human soluble KDR (D1-7).
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Protein A Chromatography.
Immunogen
Peptide of the C-terminal end of native Human soluble VEGFR-1/Flt-1
FLT1
Reactivity: Human
IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: This VEGFR1 antibody can be used at 0.5-2.0 μg/mL with the appropriatesecondary reagents to detect native Human sVEGFR-1/Flt-1. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of > 0.5 mg/mL
Buffer
PBS, pH 7.2 without preservatives or stabilizers.
Preservative
Without preservative
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulinlike sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors, including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor