DARPP32 antibody (N-Term)

Catalog No. ABIN361423

This Rabbit Polyclonal antibody specifically detects DARPP32 in WB and IHC. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for DARPP32 antibody (N-Term) (ABIN361423)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DARPP32 Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~32k DARPP-32 protein.

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primate, Xenopus

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:250 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-DARPP32 antibody (ABIN361423)

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, (2013) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: PPP1R1B

Background: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-DARPP-32 Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.

Molecular Weight: '32 kDa

Gene ID: 360616

UniProt: Q6J4I0