PKM2 antibody

Catalog No. ABIN372199

Product Details anti-PKM2 Antibody

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes M1 and M2 Isoforms of Pyruvate Kinase (Rabbit Muscle).

Purification: IgG Fractionation.

Immunogen: Pyruvate kinase purified from Rabbit muscle.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western Blot: 1/500-1/2000. Immunoprecipitation. ELISA: 1/4000-1/20000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2 (PKM2 Products)

Synonyms: CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, PKM antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, PKM antibody, Pkm antibody

Background: PKM2 (pyruvate kinase, muscle) catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. It has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 5315

NCBI Accession: NP_001193725

UniProt: P14618

Pathways: Warburg Effect