PDGFRA antibody (C-Term)

Catalog No. ABIN372897

Product Details anti-PDGFRA Antibody

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for PDGFR alpha and does not react with PDGFR beta.
Cellular Localization: Cytoplasmic and cell membrane.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: A synthetic peptide derived from C-terminal of Human PDGFR alpha.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 μg/mL for 2 hrs at RT. Immunohistochemistry on Paraffin Sections: 5 μg/mL for 45 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for10-20 min followed by cooling at RT for 20 min. Positive Control: Ovarian carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.6, 0.2 % BSA, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: CD140a / PDGFRA (PDGFRA Products)

Synonyms: AI115593 antibody, CD140a antibody, Pdgfr-2 antibody, APDGFR antibody, PDGFACE antibody, CD140A antibody, PDGFR-2 antibody, PDGFR2 antibody, RHEPDGFRA antibody, PDGFR antibody, PDGFR-A antibody, platelet derived growth factor receptor alpha antibody, platelet derived growth factor receptor, alpha polypeptide antibody, platelet-derived growth factor receptor, alpha polypeptide L homeolog antibody, platelet-derived growth factor receptor, alpha polypeptide antibody, PDGFRA antibody, Pdgfra antibody, pdgfra.L antibody

Background: Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha

Gene ID: 5156

NCBI Accession: NP_006197

UniProt: P16234

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling