BID antibody

Catalog No. ABIN377017

Product Details anti-BID Antibody

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human Bid

Characteristics: Mouse Anti-Human Bid-UNLB

Purification: Purified

Immunogen: Recombinant Bid

Clone: SB50a

Isotype: IgG2b

Application Details

Application Notes:

• Applications: ELISA - Quality tested WB - Quality tested IP
• Working Dilutions: ELISA Purified (UNLB) antibody ≤ 2 g/mL Immunoblotting Purified (UNLB) antibody ≤ 3 g/mL

Sample Volume: 0,2 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 0.2 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: Bid (BID Products)

Synonyms: FP497 antibody, 2700049M22Rik antibody, AI875481 antibody, AU022477 antibody, BID antibody, bid antibody, si:ch211-238n5.6 antibody, fp497 antibody, xbid antibody, DKFZp469E066 antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist S homeolog antibody, BID antibody, Bid antibody, bida antibody, bid.S antibody, bid antibody

Background: Members of the Bcl-2 family are pro- or antiapoptotic proteins that process one or several of the Bcl-2 homology (BH) domains. Bid is a proapoptotic, BH3-domain-only member or BH3-only protein (BOP) of the Bcl-2 family. Bid is localized in the cytosolic fraction of living cells as an inactive precursor. Activation of Bid occurs upon its cleavage by caspase-8 in the Fas and TNF- α apoptotic signaling pathways. Recent studies have suggested that Bid is also cleaved and activated by granzyme B, calpain and lysosomal extracts. Cleaved Bid translocates to mitochondria where it induces the release of cytochrome c from the intermembrane space of the mitochondria. The release of cytochrome c initiates apoptosis by activating downstream caspases through binding to Apaf-1. Bid has also been shown to interact with with Bax, another Bcl-2 family protein, to trigger a conformational change in Bax leading to the dimerization and integration of Bax into the outer mitochondrial membrane and activating the release of cytochrome c.

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity