Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Specificity
Reacts with a 65 kD protein known as CDC25A. The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase. This antibody cross reacts with rat.
Purification
Ig fraction
Immunogen
BALB/C mice were injected with purified recombinant CDC25A.