PML antibody

Catalog No. ABIN452172

Product Details anti-PML Antibody

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody recognizes normal PML proteins of lower Molecular Weight, and also recognizes the High Molecular Weight PML/RAR-alpha Chimeric protein in promyelocytic leukemia patients.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: RING finger protein 71, MYL, TRIM19, Probable transcription factor PML, Tripartitemotif-containing protein 19

Purification: Affinity Chromatography on Protein G.

Immunogen: Recombinant partial Human PML.

Clone: B3E9

Isotype: IgG1

Application Details

Application Notes: Western Blot: Use PML antibody at 1/2,000 dilution to differentiate normal PML andChimeric PML/RAR alpha molecules based on their molecular weight.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding appropriate volume of distilled water to reach a final antibody concentration of 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. After reconstitution, aliquot and store at -20 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML / RNF71 (PML Products)

Synonyms: MYL antibody, PP8675 antibody, RNF71 antibody, TRIM19 antibody, 1200009E24Rik antibody, AI661194 antibody, Trim19 antibody, RGD1562602 antibody, PML antibody, promyelocytic leukemia antibody, PML antibody, Pml antibody

Background: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals.Synonyms: MYL, Probable transcription factor PML, RING finger protein 71, TRIM19, Tripartite motif-containing protein 19

Gene ID: 5371

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus