HGF antibody

Catalog No. ABIN461958

The Mouse Monoclonal anti-HGF antibody (Clone 7-2) (ABIN461958) specifically detects HGF in WB, IHC and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for HGF antibody (ABIN461958)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7-2

Product Details anti-HGF Antibody

Specificity: Reacts with human HGF and to a lesser extent, mouse by Western Blot.

Purification: Protein G purified

Immunogen: Human HGF.

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.01 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: HGF

Background: Name/Gene ID: HGF
Subfamily: Serine S1
Family: Protease

Synonyms: HGF, DFNB39, Hepatocyte growth factor, Hepatopoeitin-A, Hepapoietin A, Hepatopoietin-A, Scatter factor), SF, F-TCF, HGFB, HPTA, Scatter factor

Gene ID: 3082

UniProt: P14210

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor