HSPD1 antibody

Catalog No. ABIN462364

Product Details anti-HSPD1 Antibody

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Hamster, Cow, Rabbit, Pig, Guinea Pig, Plant, C. elegans

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).

Purification: Protein G purified

Immunogen: Human HSP60 produced through recombinant DNA methods in E. coli.

Type of Immunogen: Recombinant protein

Clone: LK-2

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP (10 μg/mL), WB (0.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol

Preservative: PMSF

Handling Advice: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1 / HSP60 (HSPD1 Products)

Synonyms: CPN60 antibody, GROEL antibody, HLD4 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HuCHA60 antibody, SPG13 antibody, chaperonin antibody, cpn60 antibody, groel antibody, hld4 antibody, hsp60 antibody, hsp65 antibody, spg13 antibody, 60kDa antibody, Hsp60 antibody, 12 antibody, BP5 antibody, CG12101 antibody, Cpn60 antibody, Dmel\\CG12101 antibody, Dmhsp60 antibody, G62 antibody, HSP60A antibody, Hsp60A antibody, IEF16 antibody, Mmp-P1 antibody, SSP 7506 antibody, d-hsp60 antibody, hsp60A antibody, l(1)10Ac antibody, l(1)BP5 antibody, l(1)G8 antibody, l(1)HM21 antibody, l(1)L12 antibody, l(1)dp025 antibody, cb863 antibody, fa04a05 antibody, fb22d10 antibody, fi27b05 antibody, id:ibd2197 antibody, sb:cb144 antibody, wu:fa04a05 antibody, wu:fb22d10 antibody, wu:fi04a12 antibody, wu:fi27b05 antibody, MIF4 antibody, MNA2 antibody, mopA antibody, groL antibody, crpA antibody, Hspd1-30p antibody, heat shock protein family D (Hsp60) member 1 antibody, heat shock protein family D (Hsp60) member 1 S homeolog antibody, 60 kDa heat shock protein, mitochondrial antibody, heat shock protein 1 (chaperonin) antibody, Heat shock protein 60A antibody, heat shock 60 protein 1 antibody, chaperone ATPase HSP60 antibody, molecular chaperone GroEL antibody, thermosome subunit antibody, chaperonin GroEL antibody, mitochondrial chaperonin antibody, heat shock protein family D member 1 antibody, HSPD1 antibody, hspd1.S antibody, hspd1 antibody, LOC100414401 antibody, Hspd1 antibody, Hsp60A antibody, HSP60 antibody, groEL antibody, MMP_RS07785 antibody, groEl antibody, LOC100136430 antibody

Background: Name/Gene ID: HSPD1

Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL

Gene ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity