SPI1 antibody (AA 27-37)
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- Target See all SPI1 Antibodies
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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Binding Specificity
- AA 27-37
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Reactivity
- Human, Pig, Dog, Cow, Horse, Rabbit
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SPI1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SPI1 / PU.1. This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Opossum (91%) Rat, Platypus (82%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-DLYQRQTHEYY from an internal region of human SPI1 / PU.1 (NP_001074016.1, NP_003111.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Opossum (91%), Rat, Platypus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SPI1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (3.75 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- Alternative Name
- SPI1 / PU.1 (SPI1 Products)
- Synonyms
- OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody
- Background
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Name/Gene ID: SPI1
Synonyms: SPI1, 31 kDa-transforming protein, 31 kDa transforming protein, PU.1, SFPI1, SPI-1, SPI-1 proto-oncogene, SPI-A, Transcription factor PU.1 - Gene ID
- 6688
- NCBI Accession
- NP_001074016, NP_003111
- UniProt
- P17947
- Pathways
- Stem Cell Maintenance
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