IL-2 antibody

Catalog No. ABIN465313

Product Details anti-IL-2 Antibody

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: ELISA

Specificity: Human IL-2

Purification: Protein A purified

Immunogen: E. coli derived recombinant Human IL-2.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), SwELISA

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 μg/mL-5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-2 when used with biotinylated antigen affinity purified anti-human IL-2 (LS-C104522) as the detection antibody at a concentration of approximately 1-2 μg/mL. Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 2-4 ng/lane, under reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in Sterile water to a concentration on 1 mg/mL

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2 (IL2 Products)

Synonyms: IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody

Background: Name/Gene ID: IL2
Family: Interleukin

Synonyms: IL2, Aldesleukin, Interleukin 2, Lymphokine, IL-2, Interleukin-2, TCGF, T cell growth factor, T-cell growth factor

Gene ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation