IL-2 antibody
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- Target See all IL-2 (IL2) Antibodies
- IL-2 (IL2) (Interleukin 2 (IL2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This IL-2 antibody is un-conjugated
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Application
- ELISA
- Specificity
- Human IL-2
- Purification
- Protein A purified
- Immunogen
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E. coli derived recombinant Human IL-2.
Type of Immunogen: Cell extract - Isotype
- IgG1
- Top Product
- Discover our top product IL2 Primary Antibody
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- Application Notes
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Approved: ELISA (2 - 4 μg/mL), SwELISA
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 μg/mL-5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-2 when used with biotinylated antigen affinity purified anti-human IL-2 (LS-C104522) as the detection antibody at a concentration of approximately 1-2 μg/mL. Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 1-2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 2-4 ng/lane, under reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in Sterile water to a concentration on 1 mg/mL
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IL-2 (IL2) (Interleukin 2 (IL2))
- Alternative Name
- IL2 (IL2 Products)
- Synonyms
- IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody
- Background
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Name/Gene ID: IL2
Family: Interleukin
Synonyms: IL2, Aldesleukin, Interleukin 2, Lymphokine, IL-2, Interleukin-2, TCGF, T cell growth factor, T-cell growth factor - Gene ID
- 3558
- UniProt
- P60568
- Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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