PRKAA2 antibody (AA 323-372)

Catalog No. ABIN6741852

Product Details anti-PRKAA2 Antibody

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Binding Specificity: AA 323-372

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Guinea Pig, Monkey, Horse, Bat, Sheep, Zebrafish (Danio rerio), Xenopus laevis, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PRKAA2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Sheep, Dog, Bovine, Rabbit, Horse, Pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human PRKAA2 (P54646, NP_006243). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%), Stickleback (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: PRKAA2 / AMPK Alpha 2 (PRKAA2 Products)

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, AMPKA2 antibody, ampk antibody, ampk2 antibody, prkaa antibody, prkaa1 antibody, PRKAA2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit S homeolog antibody, PRKAA2 antibody, Prkaa2 antibody, prkaa2.S antibody, prkaa2 antibody

Background: Name/Gene ID: PRKAA2
Subfamily: AMPK/SNF1
Family: Protein Kinase

Synonyms: PRKAA2, ACACA kinase, Alpha2 AMPK, AMPK alpha 2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, AMPK-2, AMPKa2, AMPKalpha2, Ampk alpha2, AMPK2, PRKAA, AMPK, AMPK-alpha-2 chain, HMGCR kinase

Gene ID: 5563

NCBI Accession: NP_006243

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect