Histone 3 (H3) (acLys23) antibody

Details for Product No. ABIN4889650, Supplier: Log in to see
Antigen
  • H-3
  • histocompatibility 3
  • H3
Epitope
acLys23
164
115
115
107
92
84
82
82
81
78
74
64
60
59
58
58
43
43
41
40
37
36
36
35
33
32
32
32
30
27
24
23
22
20
20
19
19
18
18
17
17
17
16
16
16
16
16
16
15
15
15
15
12
11
11
10
10
10
9
9
9
9
9
7
7
7
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2280
1550
969
574
94
75
73
73
71
69
60
49
37
35
33
32
23
15
10
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
2079
281
18
13
1
Clonality
Polyclonal
Conjugate
Un-conjugated
75
68
63
51
50
47
39
39
39
39
39
26
21
19
8
8
8
7
7
7
7
7
7
3
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
2145
973
681
646
644
550
423
280
143
108
105
100
92
57
46
16
8
7
5
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen This Histone H3 acetylLys23 antibody was raised against a peptide including acetyllysine 23 of histone H3.
Purification None
Antigen
Alternative Name Histone H3
Background Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post- translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. In estrogen-responsive genes, histone H3 Lys18 is acetylated by CBP/p300 following estrogen stimulation, leading to acetylation of histone H3 Lys23 and methylation of Arg17 by CARM1. These events lead to transcriptional activation of the genes.
Molecular Weight 17 kDa
Gene ID 3020
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-Histone 3 (H3) (acLys23) antibody (ABIN4889650) Histone H3 acetyl Lys23 pAb tested by ChIP analysis. Chromatin IP performed using the...
Dot Blot (DB) image for anti-Histone 3 (H3) (acLys23) antibody (ABIN4889650) Histone H3 acetyl Lys23 pAb tested by dot blot analysis. Dot blot analysis was used t...
Immunofluorescence (IF) image for anti-Histone 3 (H3) (acLys23) antibody (ABIN4889650) Histone H3 acetyl Lys23 pAb tested by immunofluorescence. Staining of HeLa cells with...
Western Blotting (WB) image for anti-Histone 3 (H3) (acLys23) antibody (ABIN4889650) Histone H3 acetyl Lys23 pAb tested by Western blot. HeLa acid extract probed with His...
Product cited in: Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, 2017 (PubMed).

Larsson, Ulfhammer, Magnusson, Bergh, Lunke, El-Osta, Medcalf, Svensson, Karlsson, Jern: "Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression." in: PLoS ONE, Vol. 7, Issue 2, pp. e31573, 2012 (PubMed).

Dovey, Foster, Cowley: "Histone deacetylase 1 (HDAC1), but not HDAC2, controls embryonic stem cell differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 18, pp. 8242-7, 2010 (PubMed).

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