Nodal antibody (AA 242-253)

Catalog No. ABIN490004

Product Details anti-Nodal Antibody

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Binding Specificity: AA 242-253

Reactivity: Human, Dog, Horse, Cow, Bat, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Nodal antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NODAL.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bovine, Dog, Bat, Horse, Pig (100%) Mouse, Rat, Hamster (92%) Elephant (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (DRSQLCRKVKFQ) from internal region of human NODAL. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bovine, Dog, Bat, Horse, Pig (100%), Mouse, Rat, Hamster (92%), Elephant (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Nodal

Target: Nodal (NODAL) (Nodal Homolog (NODAL))

Alternative Name: NODAL (NODAL Products)

Synonyms: NODAL antibody, Tg.413d antibody, HTX5 antibody, nodal4 antibody, nodal4-A antibody, nr4 antibody, xnr4 antibody, Xnr-1 antibody, nodal antibody, nr1 antibody, nr1-A antibody, nodal growth differentiation factor antibody, nodal homolog (mouse) antibody, nodal antibody, nodal growth differentiation factor L homeolog antibody, nodal homolog 1 L homeolog antibody, Nodal antibody, NODAL antibody, nodal.L antibody, nodal1.L antibody

Background: Name/Gene ID: NODAL

Synonyms: NODAL, HTX5, Nodal homolog, Nodal, mouse, homolog, Nodal homolog (mouse)

Gene ID: 4838

UniProt: Q96S42

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity