FZD2 antibody (AA 267-279)

Catalog No. ABIN490012

Product Details anti-FZD2 Antibody

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Binding Specificity: AA 267-279

Reactivity: Human, Rat, Mouse, Horse, Cow, Dog, Hamster, Pig, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FZD2 / Frizzled 2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig, Opossum (100%) Rabbit, Lizard (92%) Gorilla, Xenopus, Newt (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide RYATLEHPFHCPR from an internal region of human FZD2 / Frizzled 2 (NP_001457.1). Percent identity by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig, Opossum (100%), Rabbit, Lizard (92%), Gorilla, Xenopus, Newt (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FSD2 / Frizzled 2 (FZD2 Products)

Synonyms: BG01711 antibody, CG9739 antibody, D-Fz2 antibody, D-fz2 antibody, DFZ2 antibody, DFz-2 antibody, DFz2 antibody, Dfrz2 antibody, Dfz2 antibody, Dfz[[2]] antibody, Dm Fz2 antibody, Dmel\\CG9739 antibody, FZ2 antibody, Fz2 antibody, dFz2 antibody, dfz(2) antibody, dfz2 antibody, dfz2r antibody, frz2 antibody, fz-2 antibody, fz8 antibody, zfz2 antibody, zg08 antibody, cb383 antibody, wu:fb70g09 antibody, FZD2 antibody, fzE2 antibody, hFz2 antibody, Fz-10 antibody, Fzd10 antibody, cFz-2 antibody, frizzled2 antibody, fz2 antibody, xfz2 antibody, AL033370 antibody, AW456835 antibody, Fz10 antibody, Mfz10 antibody, Mfz10a antibody, frizzled 2 antibody, frizzled class receptor 2 antibody, frizzled class receptor 2 S homeolog antibody, fz2 antibody, fzd2 antibody, FZD2 antibody, Fzd2 antibody, fzd2.S antibody

Background: Name/Gene ID: FZD2
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2

Gene ID: 2535

NCBI Accession: NP_001457

UniProt: Q14332

Pathways: WNT Signaling