MECOM antibody (AA 1205-1214)

Catalog No. ABIN490659

Product Details anti-MECOM Antibody

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Binding Specificity: AA 1205-1214

Reactivity: Human, Dog, Horse, Rabbit, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This MECOM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human MECOM. This antibody is expected to recognize all reported isoforms (NP_001098547.3, NP_005232.2, NP_004982.2, NP_001157471.1, NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse, Rabbit (100%) Elephant, Bovine, Bat, Turkey, Platypus (90%) Mouse, Pig, Opossum, Chicken, Lizard, Eye worm (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKESLHSTSH from an internal region of human MECOM (NP_001098547.3, NP_005232.2, NP_004982.2, NP_001157471.1, NP_001157472.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse, Rabbit (100%), Elephant, Bovine, Bat, Turkey, Platypus (90%), Mouse, Pig, Opossum, Chicken, Lizard, Eye worm (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 170  kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126  kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3 μg/mL.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MECOM

Target: MECOM (MDS1 and EVI1 Complex Locus (MECOM))

Alternative Name: MECOM / EVI1 (MECOM Products)

Synonyms: AML1-EVI-1 antibody, EVI1 antibody, MDS1 antibody, MDS1-EVI1 antibody, PRDM3 antibody, D630039M04Rik antibody, Evi-1 antibody, Evi1 antibody, Jbo antibody, Mds antibody, Mds1 antibody, Mds1-Evi1 antibody, Prdm3 antibody, Znfpr1b1 antibody, evi1 antibody, im:7140716 antibody, prdm3 antibody, evi-1 antibody, mecom antibody, mecom-a antibody, mecom-b antibody, xEvi-1 antibody, evi1-B antibody, MDS1 and EVI1 complex locus antibody, MDS1 and EVI1 complex locus L homeolog antibody, MDS1 and EVI1 complex locus S homeolog antibody, MECOM antibody, Mecom antibody, mecom antibody, mecom.L antibody, mecom.S antibody

Background: Name/Gene ID: MECOM

Synonyms: MECOM, AML1-EVI-1, EVI-1, EVI1, MDS1, MDS1 and EVI1 complex locus, MDS1-EVI1, Oncogene EVI1, AML1-EVI-1 fusion protein, Zinc finger protein Evi1, Myelodysplasia syndrome 1, PRDM3

Gene ID: 2122

NCBI Accession: NP_001098547, NP_005232, NP_004982, NP_001157471, NP_001157472

UniProt: Q03112