MSH2 antibody
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- Target See all MSH2 Antibodies
- MSH2 (Mismatch Repair Protein 2 (MSH2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MSH2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human
- Purification
- Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogen
- Purified His-tagged MSH2 protein was used to produced this monoclonal antibody.
- Clone
- 4C2
- Isotype
- IgG1
- Top Product
- Discover our top product MSH2 Primary Antibody
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- Application Notes
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WB 1:300-5000
FCM 1:20-100
IF() - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C for 12 months.
- Expiry Date
- 12 months
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- Target
- MSH2 (Mismatch Repair Protein 2 (MSH2))
- Alternative Name
- MSH2 (MSH2 Products)
- Synonyms
- COCA1 antibody, FCC1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, AI788990 antibody, wu:fc06b02 antibody, wu:fc13e09 antibody, zgc:55333 antibody, ATMSH2 antibody, MUTS homolog 2 antibody, msh2 antibody, mutS homolog 2 antibody, mutS homolog 2 (E. coli) antibody, MUTS homolog 2 antibody, mutS homolog 2 L homeolog antibody, MutS protein homolog 2 antibody, MSH2 antibody, Msh2 antibody, msh2 antibody, msh2.L antibody
- Background
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Synonyms: FCC1, COCA1, HNPCC, LCFS2, HNPCC1, DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Background: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
- Gene ID
- 4436
- UniProt
- P43246
- Pathways
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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