GABRB3 antibody (AA 370-433)

Catalog No. ABIN492469

Product Details anti-GABRB3 Antibody

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Binding Specificity: AA 370-433

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABRB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Specificity: Detects ~55 kDa protein. No cross-reactivity against GABAA Receptor Beta 2 or GABAA Receptor Beta 1.

Characteristics: Synonyms: GABRB-3, GABA A receptor subunit beta-3, Gamma-aminobutyric acid receptor subunitbeta-3

Purification: Protein G Chromatography.

Immunogen: Fusion protein amino acids 370-433 of Mouse GABA-A-R-Beta3 (accession numberAAB60502).

Clone: S87-25

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Beta3 GABA Receptor in 10 μg of Rat brain lysate bycolorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondaryantibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Alternative Name: GABRB3 (GABRB3 Products)

Synonyms: GABRB3 antibody, eca5 antibody, LOC100230475 antibody, ECA5 antibody, A230092K12Rik antibody, AW049585 antibody, Cp1 antibody, Gabrb-3 antibody, beta3 antibody, gamma-aminobutyric acid type A receptor beta3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, beta 3 antibody, gamma-aminobutyric acid type A receptor beta 3 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 antibody, GABRB3 antibody, gabrb3 antibody, Gabrb3 antibody

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).Synonyms: GABA A receptor subunit beta-3, GABRB-3, Gamma-aminobutyric acid receptor subunit beta-3

Gene ID: 14402

UniProt: P63080

Pathways: Sensory Perception of Sound