HGF antibody

Catalog No. ABIN492557

The Mouse Monoclonal anti-HGF antibody is suitable to detect HGF in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for HGF antibody (ABIN492557)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 07-02-14

Product Details anti-HGF Antibody

Specificity: Reacts with Human HGF and to a lesser extent, Mouse by Western Blot.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Characteristics: Synonyms: Scatter factor, Hepatopoeitin-A, HPTA

Purification: Protein G Chromatography.

Immunogen: Purified Human HGF.

Isotype: IgG2a

Application Details

Application Notes: Western Blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This HGF antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: Hepatocyte Growth Factor / HGF

Background: Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional heterodimeric polypeptide and an effector of cells expressing the Met tyrosine kinase receptor. It mediates the growth and scattering of various cell types, epithelial mesenchymal transition, the formation of tubules and lumens and promotes angiogenesis. The ligand-receptor pair has also shown to be uniquely involved in most human solid tumors and to participate in tumor development, invasion and metastasis.Synonyms: HPTA, Hepatopoeitin-A, Scatter factor

Gene ID: 3082

UniProt: P14210

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor