c-MET antibody (Activation Domain)

Catalog No. ABIN492622

This Mouse Monoclonal antibody specifically detects c-MET in WB. It exhibits reactivity toward Human.

Quick Overview for c-MET antibody (Activation Domain) (ABIN492622)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MET antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 14G9

Product Details anti-c-MET Antibody

Binding Specificity: Activation Domain

Specificity: This monoclonal MET antibody (14G9) recognizes Met activation loop.

Characteristics: Synonyms: Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met,Met proto-oncogene tyrosine kinase

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: peptide conjugated to hemocyaninEpitope: kinase activation loop.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated A431 cellsDescription: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: HGF Receptor

Background: MET is a high affinity receptor for Hepatocyte Growth Factor/Scatter Factor HGF/SF. The (HGF/SF)-(HGFR/MET) ligand-receptor system appears to have an important role in controlling interaction between mesenchymal cells and other cell types and has been implicated in the cell mitogenic response, cell motility and development. The interaction with HGF leads to autophosphorylation at multiple tyrosine residues and recruiting of several downstream signaling components (Gab1, c-cbl, PI3-kinase). The phosphorylation of Tyr 1234/1235 leads to kinase activation, and phospho-Tyr 1349 is the Gab1 binding site. Altered MET levels and/or tyrosine kinase activities have been found in different tumors, such as colon, renal, and breast cancer.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met

Gene ID: 4233

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor