Recombinant 5-Methylcytosine antibody

Catalog No. ABIN4953287

Product Details

Target: 5-Methylcytosine

Antibody Type: Recombinant Antibody

Reactivity: Please inquire

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA

Characteristics: This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.

Clone: RM231

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot Blot: 0.5-2 μg/mL,ELISA: 0.1-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,MeDIP: 0.2 μg/mL- 2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant 5mC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details

Target: 5-Methylcytosine

Alternative Name: 5-Methylcytosine (5mC)

Target Type: Chemical

Background: This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.