Recombinant 5-Methylcytosine antibody
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- Target
- 5-Methylcytosine
- Antibody Type
- Recombinant Antibody
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Methylated DNA Immunoprecipitation (MeDIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA
- Characteristics
- This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
- Purification
- Protein A purified from animal origin-free supernatant
- Immunogen
- BSA-conjugated 5-methylcytosine was used as the immunogen for this recombinant 5mC antibody.
- Clone
- RM231
- Isotype
- IgG
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- Application Notes
- The stated application concentrations are suggested starting points. Titration of the recombinant 5mC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot Blot: 0.5-2 μg/mL,ELISA: 0.1-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,MeDIP: 0.2 μg/mL- 2 μg/mL
- Restrictions
- For Research Use only
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- Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store the recombinant 5mC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- Target
- 5-Methylcytosine
- Alternative Name
- 5-Methylcytosine (5mC)
- Target Type
- Chemical
- Background
- This recombinant 5mC antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
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