GTPase NRas antibody (AA 72-101)

Catalog No. ABIN3031130

The Rabbit Polyclonal anti-GTPase NRas antibody is suitable to detect GTPase NRas in samples from Human. It has been validated for WB, ELISA, FACS and IF.

Quick Overview for GTPase NRas antibody (AA 72-101) (ABIN3031130)

Target: GTPase NRas (NRAS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-GTPase NRas Antibody

Binding Specificity: AA 72-101

Cross-Reactivity (Details): Expected species reactivity: Chicken, Mouse, Pig, Rat

Purification: Purified

Immunogen: A portion of amino acids 72-101 from the human protein was used as the immunogen for this NRAS antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NRAS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background: NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling