HNRNPD/AUF1 antibody

Catalog No. ABIN498144

Product Details anti-HNRNPD/AUF1 Antibody

Target: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPD/AUF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of hnRPD protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity-chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HNRNPD/AUF1

Target: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Alternative Name: HnRNP-D0 / HNRNPD (HNRNPD Products)

Synonyms: AUF1 antibody, AUF1A antibody, HNRPD antibody, P37 antibody, hnRNPD0 antibody, Auf1 antibody, Hnrnpd antibody, HNRNPD antibody, p37 antibody, auf1a antibody, hnrpd antibody, hnrnpd0 antibody, C230004L04 antibody, Hnrpd antibody, wu:fa28b06 antibody, zgc:162951 antibody, heterogeneous nuclear ribonucleoprotein D antibody, heterogeneous nuclear ribonucleoprotein D L homeolog antibody, HNRNPD antibody, Hnrnpd antibody, hnrnpd antibody, hnrnpd.L antibody

Background: HnRNP D/AUF1 binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3' untranslated regions of many protooncogenes and cytokine mRNAs. It also binds to double and single stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single stranded nonmonotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. It may be involved in translationally coupled mRNA turnover (referenced from swissprot).Synonyms: AU-rich element RNA-binding protein 1, AUF1, HNRPD, Heterogeneous nuclear ribonucleoprotein D0

Molecular Weight: approx. 38 kDa

Gene ID: 3184

NCBI Accession: NP_001003810

UniProt: Q14103