Nibrin antibody
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- Target See all Nibrin (NBN) Antibodies
- Nibrin (NBN)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Nibrin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- This antibody detects endogenous levels of NBS1 protein. (region surrounding Gly274)
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatography using epitope-specific immunogen
- Top Product
- Discover our top product NBN Primary Antibody
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- Application Notes
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Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Nibrin (NBN)
- Alternative Name
- Nibrin (NBN Products)
- Synonyms
- NBN antibody, AT-V1 antibody, AT-V2 antibody, ATV antibody, NBS antibody, NBS1 antibody, P95 antibody, Nbs1 antibody, im:6911679 antibody, zgc:194152 antibody, nibrin antibody, NBN antibody, nbn antibody, Nbn antibody
- Background
- The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.Synonyms: Cell cycle regulatory protein p95, NBN, NBS, NBS1, Nijmegen breakage syndrome protein 1, P95
- Molecular Weight
- approx. 95 kDa
- Gene ID
- 4683
- NCBI Accession
- NP_002476
- UniProt
- O60934
- Pathways
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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