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CDC25A antibody (pSer75)
CDC25A
Reactivity: Human, Mouse, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-CDC25A Antibody
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Target
See all CDC25A Antibodies
CDC25A
(Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
Binding Specificity
All epitopes for CDC25A antibodies
pSer75
Reactivity
All reactivities for CDC25A antibodies
Human, Mouse, Rat
Host
All hosts for CDC25A antibodies
Rabbit
Clonality
All clonalities for CDC25A antibodies
Polyclonal
Conjugate
All conjugates for CDC25A antibodies
This CDC25A antibody is un-conjugated
Application
All applications for CDC25A antibodies
Western Blotting (WB)
Specificity
This antibody detects endogenous levels of Cdc25A protein only when phosphorylated at Ser75.
Cross-Reactivity (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Purification
Affinity chromatography
Purity
> 95 % by SDS-PAGE
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Discover our top product CDC25A Primary Antibody
Alternatives
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Application Details
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Application Notes
Western Blot: 1/500 - 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Concentration
1,0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for CDC25A
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Target
CDC25A
(Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
Alternative Name
CDC25A (CDC25A Products )
Synonyms
cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody
Background
Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
Molecular Weight
approx. 59 kDa
Gene ID
993
NCBI Accession
NP_001780
UniProt
P30304
Pathways
Cell Division Cycle , Mitotic G1-G1/S Phases , M Phase
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