Cofilin antibody (pSer3)

Catalog No. ABIN498831

Product Details anti-Cofilin Antibody

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Binding Specificity: pSer3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of p-COFILN1 protein only when phosphorylated at Ser3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: Cofilin-1 (CFL1 Products)

Synonyms: cfl1 antibody, xac1 antibody, xac2 antibody, cfl antibody, cb86 antibody, sb:cb86 antibody, zgc:56501 antibody, wu:fb18a04 antibody, wu:fk77b03 antibody, 12/8 antibody, ADF antibody, ADF/cofilin antibody, Adf antibody, CG4254 antibody, Cadf antibody, D-cof antibody, D61 antibody, Dmel\\CG4254 antibody, T8 antibody, Tsr antibody, cofilin antibody, cofilin/ADF antibody, cofilin/actin depolymerizing factor homolog antibody, l(2)03101 antibody, l(2)k03101 antibody, l(2)k05633 antibody, l(2)k13409 antibody, ntf antibody, CFL antibody, AA959946 antibody, Cof antibody, CFL1 antibody, cofilin 1 (non-muscle) L homeolog antibody, cofilin 1 (non-muscle) antibody, cofilin 1 (non-muscle), like antibody, cofilin 1 antibody, twinstar antibody, cofilin antibody, cofilin 1, non-muscle antibody, Contrafreeloading antibody, cfl1.L antibody, cfl1 antibody, cfl1l antibody, CFL1 antibody, tsr antibody, COF1 antibody, TSTA_042790 antibody, Cfl1 antibody, CFL antibody

Background: Cofilin, a member of the ADF/cofilin family is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. The protein binds to actin monomers and filaments, G actin and F actin, respectively. Cofilin causes depolymerization at the minus end of filaments, thereby preventing their reassembly. The protein is known to sever actin filaments by creating more positive ends on filament fragments. Cofilin/ADF(destrin) is likely to sever F-actin without capping and prefers ADP-actin. These monomers can be recycled by profilin, activating monomers to go back into filament form again by an ADP-to-ATP exchange. ATP-actin is then available for assembly.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

Molecular Weight: approx. 19 kDa

Gene ID: 1072

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events