EIF2B4 antibody (Internal Region)

Catalog No. ABIN499136

Product Details anti-EIF2B4 Antibody

Target: EIF2B4 (Eukaryotic Translation Initiation Factor 2B, Subunit 4 Delta, 67kDa (EIF2B4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF2B4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-RKDYGSKVSL FSH

Specificity: Recognizes EIF2B4. This antibody is expected to recognize all reported isoforms (NP_751945.2, NP_001029288.1 and NP_056451.3).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Rabbit.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to (NP_751945.2, NP_001029288.1 and NP_056451.3). Genename: EIF2B4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.3-1 μg/mL. This antibody detects a approx. 60 kDa band in lysates of cell lineK562. An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EIF2B4

Target: EIF2B4 (Eukaryotic Translation Initiation Factor 2B, Subunit 4 Delta, 67kDa (EIF2B4))

Alternative Name: EIF2B4 / EIF2BD (EIF2B4 Products)

Synonyms: wu:fb54a09 antibody, zgc:110790 antibody, si:ch211-101l18.4 antibody, MGC146592 antibody, DDBDRAFT_0189067 antibody, DDBDRAFT_0232421 antibody, DDB_0189067 antibody, DDB_0232421 antibody, Eif2b antibody, EIF-2B antibody, EIF2B antibody, EIF2Bdelta antibody, EIF2B4 antibody, EIF2BD antibody, eukaryotic translation initiation factor 2B subunit delta antibody, eukaryotic translation initiation factor 2B subunit delta L homeolog antibody, eukaryotic translation initiation factor 2B, subunit 4 delta antibody, eIF2B GDP-GTP exchange factor antibody, EIF2B4 antibody, eif2b4.L antibody, eif2b4 antibody, eIF2b4 antibody, Eif2b4 antibody

Background: EIF2B4, also called eIF2B delta is part of the eIF2B complex, which contains 5 subunits. It catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP (referenced from swissprot).Synonyms: Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta

Gene ID: 8890

NCBI Accession: NP_001029288

UniProt: Q9UI10

Pathways: Methionine Biosynthetic Process