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PPP1R12A antibody (pThr853)
PPP1R12A
Reactivity: Human, Mouse, Rat, Chicken
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-PPP1R12A Antibody
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Target
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PPP1R12A
(Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
Binding Specificity
All epitopes for PPP1R12A antibodies
pThr853
Reactivity
All reactivities for PPP1R12A antibodies
Human, Mouse, Rat, Chicken
Host
All hosts for PPP1R12A antibodies
Rabbit
Clonality
All clonalities for PPP1R12A antibodies
Polyclonal
Conjugate
All conjugates for PPP1R12A antibodies
This PPP1R12A antibody is un-conjugated
Application
All applications for PPP1R12A antibodies
Western Blotting (WB), ELISA
Purpose
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MBS/MYPT1.
Cross-Reactivity
Chicken, Human, Mouse, Rat
Characteristics
Phospho-MBS/MYPT1 Thr853 Antibody detects endogenous MBS/MYPT1 only when phosphorylated at threonine853. The antibody does not recognize other myosin phosphatase regulatory subunit.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T853 of human MBS/MYPT1.
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Alternatives
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Application Details
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Application Notes
Western Blot (0.4-1 μg/mL) ELISA (0.5-1 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Handling
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Format
Liquid
Buffer
In 10 mM HEPES/KOH, 150 mM NaCl, pH 7.5 (50 % glycerol)
Storage
-20 °C
Storage Comment
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
References for anti-PPP1R12A antibody (ABIN537186)
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Lutter, Barger, Nair, Hackstadt : "Chlamydia trachomatis inclusion membrane protein CT228 recruits elements of the myosin phosphatase pathway to regulate release mechanisms. " in: Cell reports , Vol. 3 , Issue 6 , pp. 1921-31 , (2013 ) (PubMed ).
Target Details for PPP1R12A
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Target
PPP1R12A
(Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
Alternative Name
PPP1R12A / MYPT1 (PPP1R12A Products )
Synonyms
1200015F06Rik antibody, 5730577I22Rik antibody, AA792106 antibody, AV099298 antibody, D10Ertd625e antibody, Mypt1 antibody, M130 antibody, MBS antibody, MYPT1 antibody, M110 antibody, MBSP antibody, protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody, protein phosphatase 1 regulatory subunit 12A antibody, protein phosphatase 1, regulatory subunit 12A antibody, Ppp1r12a antibody, PPP1R12A antibody
Gene ID
4659
Pathways
M Phase
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