IRS1 antibody (pSer307)

Catalog No. ABIN538222

Product Details anti-IRS1 Antibody

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Binding Specificity: pSer307

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.

Specificity: Reactivity occurs against human IRS1 pS307 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA.

Cross-Reactivity: Chicken, Cow, Dog, Human, Monkey, Mouse, Pig, Rat

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S307 of human IRS1.

Application Details

Application Notes: ELISA (1:2000-1:10000)
Western Blot (1:250-1:1500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-IRS1 antibody (ABIN538222)

Danielsson, Ost, Nystrom, Strålfors: "Attenuation of insulin-stimulated insulin receptor substrate-1 serine 307 phosphorylation in insulin resistance of type 2 diabetes." in: The Journal of biological chemistry, Vol. 280, Issue 41, pp. 34389-92, (2005) (PubMed).

Andreozzi, DAlessandris, Federici, Laratta, Del Guerra, Del Prato, Marchetti, Lauro, Perticone, Sesti et al.: "Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin ..." in: Endocrinology, Vol. 145, Issue 6, pp. 2845-57, (2004) (PubMed).

Jiang, Dallas-Yang, Liu, Moller, Zhang et al.: "Salicylic acid reverses phorbol 12-myristate-13-acetate (PMA)- and tumor necrosis factor alpha (TNFalpha)-induced insulin receptor substrate 1 (IRS1) serine 307 phosphorylation and insulin resistance ..." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 180-6, (2002) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 (IRS1 Products)

Synonyms: HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody

Gene ID: 3667

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process