Huntingtin antibody (pSer421)

Catalog No. ABIN538469

Product Details anti-Huntingtin Antibody

Target: Huntingtin (HTT)

Binding Specificity: pSer421

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of HTT.

Specificity: This antibody is specific to phosphorylated human Huntingtin protein at the pS421 residue. Minimal reactivity isexpected with the non-phosphorylated form of the protein.

Cross-Reactivity: Chicken, Chimpanzee, Human, Pig

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S421 of human HTT.

Application Details

Application Notes: ELISA (1:10000-1:40000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-Huntingtin antibody (ABIN538469)

Lee, Yoshihara, Littleton: "Cytoplasmic aggregates trap polyglutamine-containing proteins and block axonal transport in a Drosophila model of Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 9, pp. 3224-9, (2004) (PubMed).

Zainelli, Ross, Troncoso, Fitzgerald, Muma: "Calmodulin regulates transglutaminase 2 cross-linking of huntingtin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 8, pp. 1954-61, (2004) (PubMed).

Trushina, Dyer, Badger, Ure, Eide, Tran, Vrieze, Legendre-Guillemin, McPherson, Mandavilli, Van Houten, Zeitlin, McNiven, Aebersold, Hayden, Parisi, Seeberg, Dragatsis, Doyle, Bender, Chacko, McMurray: "Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro." in: Molecular and cellular biology, Vol. 24, Issue 18, pp. 8195-209, (2004) (PubMed).

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: Huntingtin (HTT Products)

Synonyms: HTT antibody, hd antibody, ZHD antibody, CG9995 antibody, Dmel\\CG9995 antibody, HD antibody, Hsap\\HD antibody, Htt antibody, dHtt antibody, dhtt antibody, SLC6A4 antibody, huntington antibody, it15 antibody, htt antibody, IT15 antibody, AI256365 antibody, C430023I11Rik antibody, Hd antibody, Hdh antibody, huntingtin antibody, HTT antibody, htt antibody, LOC373520 antibody, Htt antibody

Gene ID: 3064

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport