MTOR antibody (pSer2448)

Catalog No. ABIN538478

Product Details anti-MTOR Antibody

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Binding Specificity: pSer2448

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic phosphopeptide of MTOR.

Specificity: Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. Reactivity with mouse and rat is suggested based on protein sequence homologies.

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S2448 of human MTOR.

Application Details

Application Notes: ELISA (1:15000-1:65000)
Western Blot (1:500-1:2000)
Immunohistochemistry (5.0 μg/mL)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-MTOR antibody (ABIN538478)

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, (2003) (PubMed).

Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, (2003) (PubMed).

Chen, Zheng, Foster: "Phospholipase D confers rapamycin resistance in human breast cancer cells." in: Oncogene, Vol. 22, Issue 25, pp. 3937-42, (2003) (PubMed).

Target Details for MTOR

Target: MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

Alternative Name: MTOR / FRAP1 (mTOR Products)

Synonyms: FRAP1 antibody, FRAP antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, 2610315D21Rik antibody, AI327068 antibody, Frap1 antibody, flat antibody, frap1 antibody, tor antibody, wu:fc22h08 antibody, mechanistic target of rapamycin kinase antibody, mechanistic target of rapamycin antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody, MTOR antibody, Mtor antibody, mtor antibody

Gene ID: 2475

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect