p130 antibody

Catalog No. ABIN539487

Product Details anti-p130 Antibody

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivity: Human, Chimpanzee, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p130 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of RBL2.

Specificity: This antiserum is directed against Spa310 and reacts with the Spa3120 domain of pRb2/p130 from human tissues.

Cross-Reactivity: Chimpanzee, Human, Orang-Utan

Characteristics: Antibody Reactive Against Synthetic Peptide.

Immunogen: A synthetic peptide corresponding to the Spa310 domain of human RBL2.

Application Details

Application Notes: ELISA (1:75000-1:100000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.09 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-p130 antibody (ABIN539487)

Bagella, Sun, Tonini, Abbadessa, Cottone, Paggi, De Luca, Claudio, Giordano: "A small molecule based on the pRb2/p130 spacer domain leads to inhibition of cdk2 activity, cell cycle arrest and tumor growth reduction in vivo." in: Oncogene, Vol. 26, Issue 13, pp. 1829-39, (2007) (PubMed).

Sun, Bagella, Tutton, Romano, Giordano: "From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway." in: Journal of cellular biochemistry, Vol. 102, Issue 6, pp. 1400-4, (2007) (PubMed).

DAndrilli, Masciullo, Bagella, Tonini, Minimo, Zannoni, Giuntoli, Carlson, Soprano, Soprano, Scambia, Giordano: "Frequent loss of pRb2/p130 in human ovarian carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 9, pp. 3098-103, (2004) (PubMed).

Target Details for p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Alternative Name: RBL2 / p130 (RBL2 Products)

Synonyms: RBL2 antibody, RBR2 antibody, RRB2a antibody, PRB2 antibody, RBR-2 antibody, Rb2 antibody, p130 antibody, P130 antibody, retinoblastoma-like 2 (p130) antibody, RB transcriptional corepressor like 2 antibody, related to retinoblastoma 2 antibody, RBL2 antibody, LOC541652 antibody, Rbl2 antibody

Gene ID: 5934

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases