NEDD8 antibody

Catalog No. ABIN539585

Product Details anti-NEDD8 Antibody

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit polyclonal antibody raised against full length recombinant RUB1.

Cross-Reactivity: Saccharomyces cerevisiae

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: Recombinant protein corresponding to full length Saccharomyces cerevisiae RUB1.

Application Details

Application Notes: ELISA (1:1000-1:5000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.

References for anti-NEDD8 antibody (ABIN539585)

Wang, Kang, Feng, Serino, Schwechheimer, Wei: "CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome." in: Molecular biology of the cell, Vol. 13, Issue 2, pp. 646-55, (2002) (PubMed).

del Pozo, Dharmasiri, Hellmann, Walker, Gray, Estelle: "AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response." in: The Plant cell, Vol. 14, Issue 2, pp. 421-33, (2002) (PubMed).

del Pozo, Estelle: "The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 26, pp. 15342-7, (2000) (PubMed).

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: RUB1 (NEDD8 Products)

Synonyms: NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody

Gene ID: 851717

Pathways: Ubiquitin Proteasome Pathway