FANCD2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB49367
unconjugated
Application Notes
Immunofluorescence (1:200-1:500) Western Blot (1:10000-1:20000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-5 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In Tris-glycine, 150 mM NaCl (0.05 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,-80 °C
Storage Comment
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
Bekker-Jensen, Lukas, Kitagawa, Melander, Kastan, Bartek, Lukas: "Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks." in: The Journal of cell biology, Vol. 173, Issue 2, pp. 195-206, (2006) (PubMed).
Tremblay, Pintor Dos Reis, Bradley, Galloni, Perez-Ordonez, Freeman, Brown, Gilbert, Gullane, Irish, Kamel-Reid: "Young patients with oral squamous cell carcinoma: study of the involvement of GSTP1 and deregulation of the Fanconi anemia genes." in: Archives of otolaryngology--head & neck surgery, Vol. 132, Issue 9, pp. 958-66, (2006) (PubMed).
Mukhopadhyay, Leung, Hicks, Hastings, Youssoufian, Plon: "Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia." in: The Journal of cell biology, Vol. 175, Issue 2, pp. 225-35, (2006) (PubMed).
Stiff, Reis, Alderton, Woodbine, ODriscoll, Jeggo: "Nbs1 is required for ATR-dependent phosphorylation events." in: The EMBO journal, Vol. 24, Issue 1, pp. 199-208, (2005) (PubMed).
Rothfuss, Grompe: "Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway." in: Molecular and cellular biology, Vol. 24, Issue 1, pp. 123-34, (2003) (PubMed).
Target
FANCD2
(Fanconi Anemia, Complementation Group D2 (FANCD2))