Histone 3 antibody (H3K27me3, pSer10)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K27me3, pSer10
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Dot Blot (DB)
- Purpose
- Rabbit polyclonal antibody raised against synthetic peptide of H3K27me3S10p.
- Immunogen
- A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p).
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
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ELISA (1:100-1:500)
Dot Blot (1:20000)
Western Blot (1:250)
Immunofluorescence (1:200)
Immunoprecipitation (5 µL/IP)
ChIP (1 µL/ChIP)
The optimal working dilution should be determined by the end user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In serum (0.05 % sodium azide)
- Storage
- -20 °C,-80 °C
- Storage Comment
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Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
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Dynamic regulation of histone lysine methylation by demethylases." in: Molecular cell, Vol. 25, Issue 1, pp. 1-14, (2007) (PubMed).
: "Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28." in: Oncogene, Vol. 24, Issue 21, pp. 3492-502, (2005) (PubMed).
: "Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 24217-24, (2003) (PubMed).
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Dynamic regulation of histone lysine methylation by demethylases." in: Molecular cell, Vol. 25, Issue 1, pp. 1-14, (2007) (PubMed).
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
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