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Histone 3 antibody (H3K27me3, pSer10)

H3 Reactivity: Human WB, IF, ELISA, ChIP, IP, DB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN542368
  • Target See all Histone 3 (H3) Antibodies
    Histone 3 (H3)
    Binding Specificity
    • 59
    • 47
    • 45
    • 42
    • 41
    • 37
    • 36
    • 35
    • 35
    • 35
    • 34
    • 32
    • 31
    • 29
    • 29
    • 29
    • 26
    • 25
    • 23
    • 23
    • 23
    • 22
    • 20
    • 18
    • 18
    • 17
    • 17
    • 16
    • 16
    • 16
    • 16
    • 16
    • 15
    • 14
    • 14
    • 13
    • 13
    • 12
    • 12
    • 12
    • 12
    • 12
    • 11
    • 11
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    H3K27me3, pSer10
    Reactivity
    • 1400
    • 941
    • 861
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 30
    • 29
    • 22
    • 19
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1218
    • 243
    • 8
    • 4
    Rabbit
    Clonality
    • 1121
    • 351
    • 1
    Polyclonal
    Conjugate
    • 822
    • 81
    • 63
    • 63
    • 63
    • 62
    • 62
    • 62
    • 37
    • 33
    • 23
    • 17
    • 17
    • 17
    • 17
    • 17
    • 14
    • 2
    • 1
    This Histone 3 antibody is un-conjugated
    Application
    • 1059
    • 411
    • 379
    • 313
    • 252
    • 188
    • 181
    • 179
    • 163
    • 161
    • 142
    • 127
    • 119
    • 48
    • 38
    • 34
    • 32
    • 20
    • 16
    • 14
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Dot Blot (DB)
    Purpose
    Rabbit polyclonal antibody raised against synthetic peptide of H3K27me3S10p.
    Immunogen
    A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p).
    Top Product
    Discover our top product H3 Primary Antibody
  • Application Notes
    ELISA (1:100-1:500)
    Dot Blot (1:20000)
    Western Blot (1:250)
    Immunofluorescence (1:200)
    Immunoprecipitation (5 µL/IP)
    ChIP (1 µL/ChIP)
    The optimal working dilution should be determined by the end user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In serum (0.05 % sodium azide)
    Storage
    -20 °C,-80 °C
    Storage Comment
    Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Shi, Whetstine: "Dynamic regulation of histone lysine methylation by demethylases." in: Molecular cell, Vol. 25, Issue 1, pp. 1-14, (2007) (PubMed).

    Dunn, Davie: "Stimulation of the Ras-MAPK pathway leads to independent phosphorylation of histone H3 on serine 10 and 28." in: Oncogene, Vol. 24, Issue 21, pp. 3492-502, (2005) (PubMed).

    Zheng, Hayes: "Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 24217-24, (2003) (PubMed).

  • Target
    Histone 3 (H3)
    Alternative Name
    Histone H3 (H3 Products)
    Synonyms
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
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