Flow Cytometry (1:10-50) Immunofluorescence (1:10-50) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Harris, Yang, Stein, Lucy, Brusten, Herath, Parekh, Waterfield, OHare, Neville, Page, Zvelebil: "Cluster analysis of an extensive human breast cancer cell line protein expression map database." in: Proteomics, Vol. 2, Issue 2, pp. 212-23, (2002) (PubMed).
Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, (2002) (PubMed).
Boncristiano, Majolini, DElios, Pacini, Valensin, Ulivieri, Amedei, Falini, Del Prete, Telford, Baldari: "Defective recruitment and activation of ZAP-70 in common variable immunodeficiency patients with T cell defects." in: European journal of immunology, Vol. 30, Issue 9, pp. 2632-8, (2000) (PubMed).
Target
LCK
(Lymphocyte-Specific Protein tyrosine Kinase (LCK))