MELK antibody (Middle Region)

Catalog No. ABIN5514940

Product Details anti-MELK Antibody

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MELK antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MEETPKRKGA KVFGSLERGL DKVITVLTRS KRKGSARDGP RRLKLHYNVT

Characteristics: This is a rabbit polyclonal antibody against MELK. It was validated on Western Blot.

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MELK

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MELK

Target: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Alternative Name: MELK (MELK Products)

Synonyms: MELK antibody, xmelk antibody, Melk antibody, HPK38 antibody, AI327312 antibody, MPK38 antibody, mKIAA0175 antibody, fb71b01 antibody, fe01f04 antibody, wu:fb71b01 antibody, wu:fe01f04 antibody, maternal embryonic leucine zipper kinase antibody, maternal embryonic leucine zipper kinase L homeolog antibody, MELK antibody, melk antibody, cgd5_2270 antibody, Melk antibody, melk.L antibody

Background: MELK is a serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity, phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. It acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. And it plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. It may also play a role in primitive hematopoiesis.

Alias Symbols: MELK, KIAA0175,

Protein Interaction Partner: UBC, YGR054W, Acaca, CSN1S1, MELK, MBP, LMNB1, BABAM1, ZNF622, SF3B1, EIF2S1, CDC5L, PPP1R8, HIST1H1B, CDC25B,

Protein Size: 457

Gene ID: 9833