MYLK3 antibody (N-Term)

Catalog No. ABIN5533278

Product Details anti-MYLK3 Antibody

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Binding Specificity: AA 40-69, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYLK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MYLK3

Target: MYLK3 (Myosin Light Chain Kinase 3 (MYLK3))

Alternative Name: MYLK3 (MYLK3 Products)

Synonyms: MLCK antibody, MLCK2 antibody, caMLCK antibody, D830007F02Rik antibody, RGD1305801 antibody, myosin light chain kinase 3 antibody, MYLK3 antibody, Mylk3 antibody

Background: MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Molecular Weight: 88 kDa

Gene ID: 91807

UniProt: Q32MK0

Pathways: Regulation of Muscle Cell Differentiation