GRK2 antibody (C-Term)

Catalog No. ABIN5533795

Product Details anti-GRK2 Antibody

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Binding Specificity: AA 633-660, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen: This ADRBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the C-terminal region of human ADRBK1.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: ADRBK1 (ADRBK1 Products)

Synonyms: Adrbk-1 antibody, Bark-1 antibody, GRK2 antibody, betaARK1 antibody, BARK1 antibody, BETA-ARK1 antibody, GRK-2 antibody, Grk2 antibody, beta-adrenergic receptor kinase 1 antibody, G protein-coupled receptor kinase 2 antibody, Tsp_00497 antibody, grk2 antibody, Grk2 antibody, GRK2 antibody

Background: Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Molecular Weight: 80 kDa

Gene ID: 156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling