This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human POLR2E.
POLR2E
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
For WB starting dilution is: 1:1000
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
Supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
POLR2E
(Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
MGC68604 antibody, polr2e antibody, wu:fk97g10 antibody, zgc:101098 antibody, 2410021N14Rik antibody, 25kDa antibody, AW208866 antibody, RPB5 antibody, XAP4 antibody, RPABC1 antibody, hRPB25 antibody, hsRPB5 antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog antibody, RNA polymerase II subunit E antibody, polymerase (RNA) II (DNA directed) polypeptide E, b antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody, polymerase (RNA) II (DNA directed) polypeptide E antibody, DNA-directed RNA polymerases I, II, and III subunit RPABC1 antibody, polr2e.S antibody, POLR2E antibody, polr2eb antibody, polr2e antibody, Polr2e antibody, rpb-5 antibody
Background
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.