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CD14 antibody (C-Term)

Name: CD14 antibody (C-Term)
SKU: ABIN5535579
Price: 644.88 USD
Availability: InStock

CD14 Reactivity: Human FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Western blot analysis of CD14 using rabbit polyclonal CD14 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2). (CD14 antibody (C-Term))

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (CD14 antibody (C-Term))

IHC

Western blot analysis in A549 cell line lysates (35ug/lane). (CD14 antibody (C-Term))

3 images

Catalog No. ABIN5535579

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
16

9

6

6

5

4

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 292-322, C-Term
Reactivity
229

46

30

29

28

28

25

24

22

18

15

14

10

9

8

8

8

5

2

2

2

1

1

1
Human
Host
187

62

16

3

1
Rabbit
Compatible Secondaries
Clonality
205

64
Polyclonal
Conjugate
113

28

21

16

12

5

5

4

4

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
187

97

58

47

43

42

20

19

14

13

13

12

7

4

4

3

3

3

3

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen

This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.

Isotype

Ig Fraction

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CD14 Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 1H5D8 unconjugated

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CD14 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 1A3B6 unconjugated

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CD14 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Mouse Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 Reactivity: Human FACS, WB, IHC, ELISA Host: Mouse Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal LPSR-2385 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 unconjugated

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CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP, Func Host: Mouse Monoclonal MEM-18 unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit WB, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Application Notes

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Concentration

2 mg/mL

Buffer

Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

Molecular Weight

40 kDa

Gene ID

929

UniProt

P08571

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades