DNMT1 antibody (AA 637-650)

Catalog No. ABIN492452

Product Details anti-DNMT1 Antibody

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Binding Specificity: AA 637-650

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects a ~180 kDa protein, corresponding to the apparent molecular mass of Dnmt1 on SDS-PAGE immunoblots in samples of Human and Mouse origin.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Zebrafish.
Species reactivity (tested):Human.

Characteristics: Synonyms: MCMT, HsaI, AIM, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferaseHsaI, CXXC-type zinc finger protein 9

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 637-650 of Human Dnmt1 (Gene accessionnumber NP_001370). AA Sequence: EKDDREDKENAFKR

Clone: 60B1220-1

Isotype: IgG1

Application Details

Application Notes: Western blot: 2-4 μg/mL.2 μg/mL of this antibody was sufficient for detection of Dnmt1 in 10 μg of Mouse ES cell

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1 (DNMT1 Products)

Synonyms: cb1075 antibody, cb983 antibody, dmnt1 antibody, fb05h01 antibody, mta antibody, wu:fb05h01 antibody, ADCADN antibody, AIM antibody, CXXC9 antibody, DNMT antibody, HSN1E antibody, MCMT antibody, DDM2 antibody, DECREASED DNA METHYLATION 2 antibody, DMT01 antibody, DMT1 antibody, DNA METHYLTRANSFERASE antibody, DNA METHYLTRANSFERASE 01 antibody, DNA METHYLTRANSFERASE 1 antibody, MET2 antibody, METHYLTRANSFERASE 2 antibody, METHYLTRANSFERASE I antibody, METI antibody, methyltransferase 1 antibody, Cxxc9 antibody, Dnmt antibody, Dnmt1o antibody, MTase antibody, Met-1 antibody, Met1 antibody, MommeD2 antibody, m.MmuI antibody, DNA methyltransferase 1 antibody, DNA (cytosine-5-)-methyltransferase 1 antibody, methyltransferase 1 antibody, DNA methyltransferase (cytosine-5) 1 antibody, DNMT1 antibody, dnmt1 antibody, Dnmt1 antibody, MET1 antibody

Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt 1, Dnmt 2 and Dnmt 3. Dnmt 1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt 2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt 3a and Dnmt 3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F 1, and HDAC 1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity, as well as a growth regulatory pathway that is disrupted in nearly all cancer cells. (1-6)Synonyms: AIM, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, HsaI, MCMT

Gene ID: 1786

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection