Species reactivity (expected):Mouse, Rat. Species reactivity (tested):Human.
Purification
Affinity chromatography on Protein A
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.
AGAP2
Reactivity: Human
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/40000 - 1/200000. Western Blot: 1/5000 - 1/20000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 50 μL deionized water or equivalent.
Concentration
2.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
AGAP2
(ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))
AGAP2 (also known as centauringamma1, ARF-GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2, Phosphatidylinositol-3-kinase enhancer, PIKE, GTP-binding and GTPase activating protein 2 and GGAP2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. AGAP2 protein binds to and activates the protein kinase Akt and prevents apoptosis of cancer cells. AGAP2 also increases motility and invasive activity of cancer cells. AGAP2 may serve as a diagnostic and therapeutic target of human cancer.Synonyms: Arf-GAP GTPase ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, GGAP2, GTP-binding and GTPase-activating protein 2, KIAA0167, PIKE, Phosphatidylinositol-3-kinase enhancer