POLK antibody (AA 817-830)

Catalog No. ABIN401173

Product Details anti-POLK Antibody

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Binding Specificity: AA 817-830

Reactivity: Human, Chimpanzee, Orang-Utan

Host: Goat

Clonality: Polyclonal

Conjugate: This POLK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against POLK protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Orangutan.
Species reactivity (tested):Human.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa)

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2000 - 1/10000. Western Blot: 1/500 - 1/3000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.4 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: DNA Polymerase kappa (POLK Products)

Synonyms: DINB1 antibody, DINP antibody, POLQ antibody, Dinb1 antibody, POLK antibody, si:ch211-254o18.3 antibody, DNA polymerase kappa antibody, polymerase (DNA directed) kappa antibody, polymerase (DNA directed), kappa antibody, polymerase (DNA directed) kappa L homeolog antibody, POLK antibody, Polk antibody, polk antibody, Tb11.01.0080 antibody, Tb11.01.0010 antibody, Tb11.01.0020 antibody, Tb11.01.0030 antibody, Tb11.01.0040 antibody, Tb11.01.0050 antibody, Tb11.12.0001 antibody, Tb11.12.0002 antibody, Tb11.12.0003 antibody, polk.L antibody, polk-1 antibody

Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.Synonyms: DINB protein, DINB1, DINP, POLK

Gene ID: 51426

NCBI Accession: NP_057302

UniProt: Q9UBT6

Pathways: DNA Damage Repair