c-Rel antibody (C-Term)

Catalog No. ABIN116522

Product Details anti-c-Rel Antibody

Target: c-Rel (REL proto-oncogene (c-Rel))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-Rel antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Specificity: Antibody NFkB cRel may react non-specifically with other proteins. Control peptide (code R1010CP) will compete only with the specific reaction of antiserum with the NFkB cRel subunit.

Purification: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against NFkB cRel at a dilution of 1: 1000 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NFkB cRel is suitable for thedetection by immunoblot of human NFkB cRel. No reaction was observed against theanologous Mouse protein. This product was also tested in a gel supershift assay and foundto be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 l perassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Target Details for c-Rel

Target: c-Rel (REL proto-oncogene (c-Rel))

Alternative Name: REL (c-Rel Products)

Synonyms: C-Rel antibody, c-Rel antibody, NFkB antibody, c-rel antibody, zgc:100833 antibody, Xrel2 antibody, Xrel3 antibody, rel antibody, rel-A antibody, rel2 antibody, rel3 antibody, v-rel antibody, xrel antibody, REL proto-oncogene, NF-kB subunit antibody, reticuloendotheliosis oncogene antibody, RELA proto-oncogene, NF-kB subunit antibody, v-rel avian reticuloendotheliosis viral oncogene homolog antibody, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antibody, ribonuclease A family member 2 pseudogene antibody, REL antibody, Rel antibody, Rela antibody, rel antibody, rel.L antibody, ECRP antibody

Background: NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFκB bound to IκB. NFκB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DANN binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFκB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IκB-α. IκB-α binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: C-Rel proto-oncogene protein, c-Rel

Gene ID: 5966

NCBI Accession: NP_002899

UniProt: Q04864