The antibody is directed against Human HER3. It can detect HER3 from Human, Mouse and Rat sources. Reactivity of this antibody with HER3 from other species is unknown.
Purification
Protein A Chromatography followed by cross adsorption against GST and extensive dialysis against the buffer stated below.Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit.
Immunogen
This whole serum was prepared by repeated immunizations with a GST-HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.
ERBB3
Reactivity: Human
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Anti-HER3 antibody is specifically designed for Immunoprecipitation and can be used forImmunoblotting in conjunction with a detection antibody like anti-phosphotyrosine. Reactivity in other assays is likely, but has not been determined. Recognition of HER3 isindependent of the phosphorylation status of the molecule. Human prostate cell lines(LNCap) or human breast cell line (MDA-453) are typically used as positive control sources. Recommended Dilutions: Immunoprecipitation: Use approximately 5 μL of the antibody. The Immunoprecipitation
Restrictions
For Research Use only
Concentration
5.74 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M potassium phosphate, 0.12 M sodium chloride, pH 7.2 with 0.01 % Sodium Azide as preservative without Stabilizers.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
The human Epidermal Growth Factor Receptor (EGFR) family is a family of receptor tyrosine kinases consisting of 4 members: EGFR, HER2, HER3 and HER4. These receptors are expressed on a wide variety of tissue types and have been implicated in many human cancers, including breast, gastric, colon and prostate. Strong expression of both HER3 and HER2 (also known as p185neu) by tumor cells can be associated with poor outcome for patients. The ligand for HER3 and HER4 is called Neu Differentiation Factor (NDF) or Heregulin. In some instances, antibodies against these receptors prove effective in combination therapy regimens for treatment of the disease. Signaling through the EGFR family involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins. Heregulin binding and tyrosine phosphorylation by HER3 or HER4 is enhanced in the presence of HER2. Structurally the members of the EGFR family are 1210 to 1342 amino acids in length with approximately 650 amino acids of the extracellular domain being heavily glycosylated. The extracellular domains show up to 70 % sequence homology.Synonyms: ERBB-3, HER-3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB-3