RPS6KA1 antibody (C-Term)

Catalog No. ABIN5596747

Product Details anti-RPS6KA1 Antibody

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross reactivity with RSK1 proteins from human, rat and mouse. Partial reactivity may occur against RSK2 and RSK3 but has not been tested. Reactivity against homologues from other sources is not known.

Purification: This affinity-purified antibody is directed against the human RSK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human RSK1 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 88 kDa in size corresponding to RSK1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:1000

ELISA Dilution: 1:2,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Stabilizer: 50 % (v/v) Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Alternative Name: p90 RSK1 (RPS6KA1 Products)

Synonyms: HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Rsk1 antibody, p90rsk antibody, rsk antibody, RPS6KA antibody, hu-1 antibody, mapkapk1a antibody, rsk1 antibody, wu:fc56h07 antibody, zgc:152654 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase polypeptide 1 antibody, ribosomal protein S6 kinase A1-like antibody, ribosomal protein S6 kinase A1 L homeolog antibody, ribosomal protein S6 kinase a, polypeptide 1 antibody, RPS6KA1 antibody, Rps6ka1 antibody, RPS6KA1L antibody, rps6ka1.L antibody, rps6ka1 antibody

Background:

Synonyms: 90 kDa ribosomal protein S6 kinase 1 antibody, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody, dJ590P13.1 antibody, EC 2.7.11.1 antibody, HU 1 antibody

Background: RSK1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsk's appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.

Gene Name: RPS6KA1, RSK1

Gene ID: 6195

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades