PFKM antibody (HRP)
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- Target See all PFKM Antibodies
- PFKM (phosphofructokinase, Muscle (PFKM))
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Reactivity
- Rabbit
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PFKM antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.
- Purification
- Fructose-6-Phosphate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].
- Immunogen
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Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product PFKM Primary Antibody
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- Application Notes
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Application Note: Anti-Fructose-6-Phosphate Kinase antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PFKM (phosphofructokinase, Muscle (PFKM))
- Alternative Name
- Fructose-6-Phosphate Kinase (PFKM Products)
- Synonyms
- GSD7 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, AI131669 antibody, PFK-A antibody, PFK-M antibody, Pfk-4 antibody, Pfk4 antibody, Pfka antibody, Pfkx antibody, Pfk-M antibody, M-PFK antibody, PFKM antibody, pfkm antibody, zgc:92344 antibody, pfk antibody, im:7137110 antibody, wu:fc58g11 antibody, phosphofructokinase, muscle antibody, phosphofructokinase, platelet antibody, phosphofructokinase, muscle a antibody, phosphofructokinase, muscle S homeolog antibody, phosphofructokinase, muscle b antibody, PFKM antibody, Pfkm antibody, PFKP antibody, pfkma antibody, pfkm.S antibody, pfkmb antibody
- Background
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Synonyms: 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody, HRP
Background: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.
Gene Name: PFKM
- Gene ID
- 100345647
- UniProt
- P00511
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
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